H. Stuppner et M. Ganzera, APPLICATION OF BETA-CYCLODEXTRIN FOR THE ANALYSIS OF THE MAIN ALKALOIDS FROM CHELIDONIUM-MAJUS BY CAPILLARY ELECTROPHORESIS, Journal of chromatography, 717(1-2), 1995, pp. 271-277
Citations number
7
Categorie Soggetti
Chemistry Analytical","Biochemical Research Methods
Capillary zone electrophoresis (CZE) with multi-wavelength detection w
as used for the separation of the main alkaloids from Chelidonium maju
s. Optimum separation was achieved with a fused-silica capillary tube
(70 cm x 50 mu m I.D.) and a running electrolyte at pH 5.5 prepared fr
om 40 mM citric acid and 80 mM Na2HPO4 solutions. Addition of beta-cyc
lodextrin (12.5 mmol/l) to the buffer system was found to be essential
for the separation. The applied voltage was 25 kV and the capillary t
emperature was kept constant at 25 degrees C.