INHIBITION OF FIBROBLAST PROLIFERATION BY HUMAN IRIS PIGMENT EPITHELIAL-CELLS IN-VITRO - PRELIMINARY-RESULTS

Authors
Citation
Zj. Fan et al., INHIBITION OF FIBROBLAST PROLIFERATION BY HUMAN IRIS PIGMENT EPITHELIAL-CELLS IN-VITRO - PRELIMINARY-RESULTS, Graefe's archive for clinical and experimental ophthalmology, 234(1), 1996, pp. 64-66
Citations number
9
Categorie Soggetti
Ophthalmology
ISSN journal
0721832X
Volume
234
Issue
1
Year of publication
1996
Pages
64 - 66
Database
ISI
SICI code
0721-832X(1996)234:1<64:IOFPBH>2.0.ZU;2-X
Abstract
Background: The interaction between different cells plays an important role in many physiological and pathological processes. Since the prol iferation of fibroblasts is very much involved in the pathogenesis of eye diseases such as proliferative vitreoretinopathy, the failure of f iltration in glaucoma surgery, etc., we attempted to ascertain whether iris pigment epithelial cells (IPE) have some modulating effect on fi broblast proliferation. Methods: Human IPE were explanted and the thir d-passage culture was transferred into serum-fi ee RPMI-1640 medium. A fter 48 h of further incubation, the medium was collected and submitte d to centrifugation; the supernatant was used as the conditioned mediu m of IPE (IPE-CM). Cultured fibroblasts from Tenon's capsule were seed ed in a 96-well plate and incubated with IPE-CM in different concentra tions. The proliferation of fibroblasts was estimated by thymidine inc orporation and cell counting. Results: The incorporation of tritiated thymidine by fibroblasts was reduced to 56.68% of baseline with 1:16 d iluted IPE-CM and to 13.63% and 8.20%, respectively, with 1:8 and 1:2 diluted IPE-CM. These findings were in good accordance with the result s of cell counting! performed in parallel. SDS-PACE of IPE-CM revealed two specific bands with molecular weight 65 kDa and 40 kDa. Conclusio n: IPE-CM showed an obvious dose-dependent inhibitory effect on fibrob last proliferation and was presumed to contain some active factors con tributing to this effect.