MODULATION OF CISPLATIN CYTOTOXICITY BY HUMAN RECOMBINANT INTERFERON-GAMMA IN HUMAN OVARIAN-CANCER CELL-LINES

Cytotoxic interactions between recombinant human interferon-gamma (IFN gamma) and cisplatin have been studied in six ovarian cell lines (IGR OV1, NIHOVCAR3, SKOV3, OVCCR1, 2008 and its cisplatin resident variant 2008/C13). Studies were performed using a cell survival assay. Resul ts were assessed using median effect analysis. Synergy between these t wo drugs was observed in cell lines sensitive to IFN gamma, whatever t heir relative sensitivity or resistance to cisplatin, suggesting that IFN gamma enhances the cytotoxic activity of cisplatin. This interacti on is not due to an increase in platinum accumulation in cells. This c ombination of drugs should be evaluated against human ovarian cancer x enografts in nude mice before its use in clinical practice.