MOLECULAR-CLONING AND NUCLEOTIDE-SEQUENCE OF THE 1,2-ALPHA-D-MANNOSIDASE GENE, MSDS, FROM ASPERGILLUS-SAITOI AND EXPRESSION OF THE GENE IN YEAST-CELLS

Authors
INOUE T YOSHIDA T ICHISHIMA E
Citation
T. Inoue et al., MOLECULAR-CLONING AND NUCLEOTIDE-SEQUENCE OF THE 1,2-ALPHA-D-MANNOSIDASE GENE, MSDS, FROM ASPERGILLUS-SAITOI AND EXPRESSION OF THE GENE IN YEAST-CELLS, Biochimica et biophysica acta. Protein structure and molecular enzymology, 1253(2), 1995, pp. 141-145
Citations number
25
Categorie Soggetti
Biology,Biophysics
Journal title
Biochimica et biophysica acta. Protein structure and molecular enzymology → ACNP
ISSN journal
01674838
Volume
1253
Issue
2
Year of publication
1995
Pages
141 - 145
Database
ISI
SICI code
0167-4838(1995)1253:2<141:MANOT1>2.0.ZU;2-O
Abstract
A full-length cDNA encoding 1,2-alpha-D-mannosidase (EC 3.2.1.113) fro m Aspergillus saitoi was cloned. Analysis of the 1718 bp 'nucleotide s equence of the cDNA revealed a single open reading frame with 1539 nuc leotides of 1,2-alpha-D-mannosidase gene, msdS. The predicted amino-ac id sequence of 1,2-alpha-D-mannosidase consists of 513 residues with a molecular mass of 55,767 and is 70%, 26% and 35% identity with those of Penicillium citrinum 1,2-alpha-D-mannosidase, yeast cr-mannosidase, and mouse alpha-mannosidase. The cDNA of the msdS gene has been clone d and expressed in yeast cells. To identify the activity of expression product 2-O-alpha-mannopyranosyl-alpha-mannopyranoside(Man alpha 1 -- > 2Man-OMe) was used as a substrate at pH 5.0.