Ja. Gwinnett et al., COMPARISON OF 3 METHODS OF CRITICAL-EVALUATION OF MICROLEAKAGE ALONG RESTORATIVE INTERFACES, The Journal of prosthetic dentistry, 74(6), 1995, pp. 575-585
This in vitro study compared microleakage along the dentin-restorative
interface by use of a conventional single-surface and two stereoscopi
cal protocols. The extent of microleakage in class V cylindric cavitie
s that were restored with Scotchbond 2/P-50 was assessed after thermoc
ycling and silver staining were performed. Specimens were randomized i
nto three groups of 10 teeth for the three different protocols to be i
nvestigated. The categories were group 1, a single longitudinal sectio
n through the midline of the restoration, group 2, a multiple-surface
protocol based on computer reconstruction of digitized images from mul
tiple serial abrasions, and group 3, a clearing protocol based on imme
rsion of demineralized specimens in methyl salicylate. The severity of
microleakage for all groups was recorded on an ordinal scale and base
d on linear leakage length. The median leakage score for groups 1 thro
ugh 3 was 2, 4, and 4, respectively. When comparisons were performed w
ith nonparametric statistical analysis, both the clearing and the mult
iple-surface protocols recorded significantly more severe leakage than
those detected from a conventional, single longitudinal, midline sect
ioning technique, (x(2) = 8.595, p < 0.02). Furthermore microleakage p
atterns generated from the two stereoscopical protocols revealed exten
sive microleakage that advanced unilaterally or bilaterally through th
e dentin-restorative interface along the embrasure regions into the ax
ial wall of the restorations. No significant difference was noted betw
een the two stereoscopical protocols (Q = 0.260, p > 0.05), which sugg
ested that the clearing protocol, which is less labor-intensive than t
he multiple-surface protocol, may be recommended for future comparativ
e microleakage studies that involve the dentin-restorative interface.