SCANNING ELECTRON-MICROSCOPY AND ENERGY-DISPERSIVE SPECTROSCOPY ANALYSIS OF CALCIOTRAUMATIC LINES IN RAT LABIAL DENTIN AFTER ACUTE EXPOSURETO STRONTIUM CHLORIDE

Rats were given strontium chloride (SrCl2) intraperitoneally at a dose of 500 mg/kg. The upper incisors were removed after injection of stro ntium. These incisors were studied by scanning electron microscopy ene rgy dispersive spectroscopy analysis (SEM-EDS) and light microscopy to examine the calciotraumatic lines of strontium in the rat incisor lab ial dentin. At 24 hours after injection of strontium, the calciotrauma tic response was observed in the predentin using hematoxylin and eosin (H-E) staining. At 5 days, three layers of calciotraumatic lines were present in the labial dentin using an H-E staining and backscattered electron imaging in the SEM. The external layer consisted of unmineral ized dentin, the intermediate layer of relatively unmineralized dentin , and the internal layer of unmineralized dentin. By SEM-EDS analysis, strontium was detected in these layers. The elemental dot map showed that the external and internal unmineralized layers had a low calcium content. The magnesium concentration was higher in the internal unmine ralized layer than the external unmineralized layer.