HIGH-PERFORMANCE ANION-EXCHANGE CHROMATOGRAPHY OF CARBOHYDRATES USINGA NEW RESIN AND PULSED AMPEROMETRIC DETECTION

This paper reports the results of a study on the use of a new polymer- based, strong anion-exchange, stationary phase for rapid and selective separation of carbohydrates and related compounds by high-pH, anion-e xchange chromatography with pulsed amperometric detection. The new ads orbent has been obtained by direct nitration of 2.8 mu m, spherical no n-porous highly crosslinked, styrene-divinylbenzene copolymer beads, f ollowed by reduction of superficially introduced nitro groups with nas cent hydrogen and quaternization of the resultant amino groups with io domethane. It is reported that by optimizing the ionic strength of the mobile phase, columns packed with the new anion-exchanger can be succ essfully employed to separate, either in isocratic or gradient elution mode, oligosaccharides, positional isomers of gluco-disaccharides, as well as uronic acids and sugar monophosphates.