OPTIMIZATION OF A CHROMATOGRAPHIC METHOD FOR THE GRAM-SCALE PREPARATIVE FRACTIONATION OF SOYBEAN PHOSPHOLIPIDS

Citation
B. Demeulenaer et al., OPTIMIZATION OF A CHROMATOGRAPHIC METHOD FOR THE GRAM-SCALE PREPARATIVE FRACTIONATION OF SOYBEAN PHOSPHOLIPIDS, Chromatographia, 41(9-10), 1995, pp. 527-531
Citations number
13
Categorie Soggetti
Chemistry Analytical","Biochemical Research Methods
Journal title
ISSN journal
00095893
Volume
41
Issue
9-10
Year of publication
1995
Pages
527 - 531
Database
ISI
SICI code
0009-5893(1995)41:9-10<527:OOACMF>2.0.ZU;2-Z
Abstract
To enable the study of the functional properties of pure soybean phosp holipids, a chromatographic method was developed for the preparative f ractionation of soybean lecithin. A coarse and irregularly shaped sili ca gel was used as the stationary phase, whereas the mobile phase cons isted of three mixtures of hexane, 2-propanol and water of increasing polarity. These solvents were included in a step gradient, formed by a n isocratic pump connected to a solvent changer.To reduce solvent cons umption, the procedure was optimised using a method development column . The solvent program and the sample load were shown to affect mainly the purity and recovery of phosphatidylinositol.