QUANTITATIVE-DETERMINATION OF TOXIC MONO-ORTHO AND NON-ORTHO POLYCHLORINATED-BIPHENYLS IN COD-LIVER OIL AFTER SELECTIVE LIQUID-CHROMATOGRAPHIC SEPARATION

By application of a chromatographic column filled with Supelclean ENVI -Carb/Celite and elution with three solvents of different polarity thr ee PCB fractions were obtained. Fraction A contained poly-ortho PCBs, Fraction B mono-ortho PCBs, and Fraction C non-ortho PCBs. The Supelcl ean ENVI-Carb/Celite column was used in combination with a sample prep aration procedure for pre-cleaning of acid-stable chlorinated hydrocar bons such as DDT and its metabolites, HCH isomers, and regulation-rele vant PCB congeners. The method was optimized using standard solutions of 55 PCB congeners, 8 chlorinated pesticides and contaminated cod liv er oil samples. The influence of traces of remaining matrix on the elu tion profile of the organochlorine compounds on Supelclean ENVI-Carb/C elite was observed. Quantitation was carried out by GC-ECD with fused silica capillary columns of different polarity.