SEPARATION AND DETERMINATION OF 10-HYDROXY-2-DECENOIC ACID IN ROYAL JELLY BY CAPILLARY ELECTROPHORE

The application of capillary electrophoresis (CE) to the separation an d determination of the active ingredient, 10-hydroxy-2-decenoic acid, in royal jelly with direct on-column UV detection at 214 nm is describ ed. Using a cathodic injection and anodic detection scheme, 10-hydroxy -2-decenoic acid (10-HDA) was separated and detected in less than 10 m in in a fused silica capillary column with a phosphate buffer at pH 7. 3 with an applied voltage of 20 KV followed by direct UV detection. Th e use of cetyltrimethylammonium bromide (CTAB) as electroosmotic flow modifier allows the rapid separation of 10-HDA from other constituents in royal jelly by reversing the direction of electroosmotic flow. The influence of organic solvents in the electrolyte on separation select ivity is also discussed.