PHYLOGENETIC-RELATIONSHIPS BETWEEN 6 SPECIES OF PHASEOLUS AND VIGNA AS DETERMINED BY RFLP ANALYSIS OF RIBOSOMAL DNA

Phylogenetic relationships between four Phaseolus (P. angularis, P. ca lcaratus, P. coccineus and P. vulgaris (including six cultivars)) and two Vigna (V. sesquipedalis and ii. sinensis) species were studied by restriction fragment length polymorphism (RFLP) analysis using 15 rest riction enzymes and two ribosomal DNA fragments from lice and mung bea n as probes. Based on the RFLP data, phylogenetic trees were construct ed by the distance matrix and maximum parsimony methods. The two trees constructed by the distance matrix method and one tree constructed by the maximum parsimony method had congruent topology and recognized th ree clusters: one containing V. sesquipedalis and V. sinensis; a secon d containing P. angularis; and a third containing P. calcaratus, P. co ccineus and six cultivars of P. vulgaris. Grouping of the six species based on the molecular taxonomic data obtained is compared with groupi ng based on previously reported cytological data.