Mg. Niemialtowski et al., QUANTITATIVE STUDIES ON CD4-LYMPHOCYTE RESPONSES AGAINST HERPES-SIMPLEX VIRUS TYPE-1 IN NORMAL AND BETA-2-M DEFICIENT MICE( AND CD8+ CYTOTOXIC T), Immunobiology, 190(3), 1994, pp. 183-194
Against herpes simplex virus type 1 (HSV-1) infected target cells, eff
ector T cells of both CD4(-) CD8(+) and CD4(+) CD8(-) phenotypes occur
in both man and mice. In man, the CD4(+) CD8(-) phenotype may dominat
e the response; but the situation in the mouse is unclear. In this rep
ort, several approaches are used to document the existence of HSV-1-sp
ecific cytotoxic T lymphocytes (CTL) of both phenotypes and to quantit
ate the frequency of their CTL precursors (CTL-p) in acutely HSV-1 inf
ected animals. Evidence that CD4(+) CD8(-) CTL occur was confirmed wit
h beta 2-m(-) mice lacking CD8(+) T cells and which generated CTL capa
ble only of lysing MHC class II infected target cells. Comparisons of
frequencies of CD4(+) CD8(-) and CD4- CD8(+) CTL-p showed the former t
o be 2-3-fold more frequent. The beta 2-m(-) mice generated only CD4() CD8(-) CTL-p and their frequencies approximately equally the CD4(+)
CD8(-) CTL-P frequency in intact infected mice. Our results are discus
sed in terms of the value of the mouse model to study HSV-1 immunobiol
ogy.