EXPRESSION OF MESSENGER-RNA FOR 55-KDA AND 75-KDA TUMOR-NECROSIS-FACTOR (TNF) RECEPTORS IN MOUSE CEREBROVASCULAR ENDOTHELIUM - EFFECTS OF INTERLEUKIN-1-BETA, INTERFERON-GAMMA AND TNF-ALPHA ON CULTURED-CELLS

The interactions between tumor necrosis factor-alpha (TNF-alpha) and i ts receptors have been implicated to play an important role in the pat hogenesis of experimental autoimmune encephalomyelitis (EAE). The effe cts of cytokines on the steady stare levels of TNF receptor (TNFr) mRN A in cerebrovascular endothelial cells (CVE) cultured from EAE-suscept ible (SJL/J) and EAE-resistant (BALB/c) mice were examined. Interferon -gamma and interleukin-1 beta up-regulated the levels of both the 55-k Da and 75-kDa TNFr mRNA, while TNF-alpha had no effect. No differences were observed in cytokine-induced TNFr mRNA levels between BALB/c and SJL/J CVE that might explain differences in EAE susceptibility.