SEPARATION OF LARGE DNA-MOLECULES FROM PLEUROTUS-CORNUCOPIAE BY PULSED-FIELD GEL-ELECTROPHORESIS

Authors
TAMAI Y YOKONO T SUNAGAWA M KOJIMA Y MIURA K
Citation
Y. Tamai et al., SEPARATION OF LARGE DNA-MOLECULES FROM PLEUROTUS-CORNUCOPIAE BY PULSED-FIELD GEL-ELECTROPHORESIS, Mokuzai Gakkaishi, 41(10), 1995, pp. 952-955
Citations number
14
Categorie Soggetti
Materials Science, Paper & Wood
Journal title
Mokuzai Gakkaishi → ACNP
ISSN journal
00214795
Volume
41
Issue
10
Year of publication
1995
Pages
952 - 955
Database
ISI
SICI code
0021-4795(1995)41:10<952:SOLDFP>2.0.ZU;2-C
Abstract
An optimum condition for the separation of large DNA molecules from Pl eurotus cornucopiae (Paulet) Rolland var. citrinopileatus (Sing.) Ohir a was investigated by using the pulsed-field gel electrophoresis (PFGE ) method. A clear separation of the DNA molecules was achieved by, a c ombination of switching intervals and running voltages. Nine and seven bands were separated from the dikaryon and the monokaryon, respective ly, within 96 h. They ranged in size from 1.7 to 3.7 Mb. Their genome sizes were estimated as about 55.2 Mb and 27.8 Mb, respectively.