EXPRESSION OF THE CYANIDE HYDRATASE ENZYME FROM FUSARIUM-LATERITIUM IN ESCHERICHIA-COLI AND IDENTIFICATION OF AN ESSENTIAL CYSTEINE RESIDUE

Authors
BROWN DT TURNER PD OREILLY C
Citation
Dt. Brown et al., EXPRESSION OF THE CYANIDE HYDRATASE ENZYME FROM FUSARIUM-LATERITIUM IN ESCHERICHIA-COLI AND IDENTIFICATION OF AN ESSENTIAL CYSTEINE RESIDUE, FEMS microbiology letters, 134(2-3), 1995, pp. 143-146
Citations number
11
Categorie Soggetti
Microbiology
Journal title
FEMS microbiology letters → ACNP
ISSN journal
03781097
Volume
134
Issue
2-3
Year of publication
1995
Pages
143 - 146
Database
ISI
SICI code
0378-1097(1995)134:2-3<143:EOTCHE>2.0.ZU;2-E
Abstract
The filamentous fungus Fusarium lateritium is cyanide tolerant, due pa rtly to the induction of the enzyme cyanide hydratase in the presence of cyanide. This enzyme catalyses the hydration of cyanide to formamid e. The expression in Escherichia coli of a cDNA clone encoding cyanide hydratase is described. The cDNA cloned was expressed as a transcript ional fusion in the expression vector pKK233-2 and a high level of act ivity of cyanide hydratase was detected in E. coli. Site-directed muta genesis of the cys-163 residue inactivated the enzyme.