CLONING AND SEQUENCING OF A GENE ENCODING PYRUVATE-KINASE FROM SCHIZOSACCHAROMYCES-POMBE - IMPLICATIONS FOR QUATERNARY STRUCTURE AND REGULATION OF THE ENZYME

A cDNA encoding pyruvate kinase from Schizosaccharomyces pombe has bee n isolated from a lambda ZAPII library. This cDNA was sequenced and fo und to contain an open reading frame of 1524 nucleotides, giving a pre dicted protein subunit M(r) of 55470. The sequence shows a high degree of identity with other pyruvate kinase sequences, with residues impli cated in the binding of substrate and metal ion co-factors conserved. However, there are significant differences in the putative subunit int erface and effector binding regions which may account for the unusual quaternary structure and regulatory properties of the S. pombe enzyme.