Mc. Menendez et al., CLONING AND EXPRESSION OF THE MYCOBACTERIUM-FORTUITUM SUPEROXIDE-DISMUTASE GENE, FEMS microbiology letters, 134(2-3), 1995, pp. 273-278
In this paper we report the cloning, sequencing and expression of the
superoxide dismutase (sod) gene from Mycobacterium fortuitum. A single
gene was found to code for superoxide dismutase activity with its ide
ntity being confirmed by expression in M. aurum. The amino acid sequen
ce was found to be similar to that of superoxide dismutases of several
other origins. A region downstream of the sod gene also showed simila
rities to the corresponding sequences of the two main mycobacterial pa
thogens: M. leprae and M. tuberculosis, Analysis of enzymatic activity
showed this enzyme in M. fortuitum required manganese as cofactor.