IDENTIFICATION OF STREPTOMYCES-OLIVACEUS TU-2353 GENES INVOLVED IN THE PRODUCTION OF THE POLYKETIDE ELLORAMYCIN

Authors
DECKER H ROHR J MOTAMEDI H ZAHNER H HUTCHINSON CR
Citation
H. Decker et al., IDENTIFICATION OF STREPTOMYCES-OLIVACEUS TU-2353 GENES INVOLVED IN THE PRODUCTION OF THE POLYKETIDE ELLORAMYCIN, Gene, 166(1), 1995, pp. 121-126
Citations number
29
Categorie Soggetti
Genetics & Heredity
Journal title
Gene → ACNP
ISSN journal
03781119
Volume
166
Issue
1
Year of publication
1995
Pages
121 - 126
Database
ISI
SICI code
0378-1119(1995)166:1<121:IOSTGI>2.0.ZU;2-K
Abstract
The genes for the production of elloramycin (ELM) from Streptomyces ol ivaceus (So) Tu2353 were cloned using a polyketide synthase gene probe from the tetracenomycin pathway. A cosmid clone (16F4) isolated from a gene library of So Tu2353 conferred tetracenomycin C and ELM resista nce to S. lividans TK64 and complemented a mutation in So Tu2353R. Int roduction of cosmid 16F4 into S. lividans TK64 resulted in the product ion of 8-demethyl-tetracenomycin C, an intermediate of ELM biosynthesi s.