ESCHERICHIA-COLI EXPRESSION VECTORS CONTAINING A PROTEIN-KINASE RECOGNITION MOTIF, HIS(6)-TAG AND HEMAGGLUTININ EPITOPE

Escherichia coli expression vectors, based on the pET system, were con structed to allow fusion of a protein kinase (PK) recognition motif, a hemagglutinin (HG) epitope-tag and a His(6)-tag at the N-terminal por tion of a protein of interest, The fusion proteins, that result from e xpression using these vectors, can be phosphorylated in vitro using cA MP-dependent PK, immunoprecipitated using monoclonal antibody against the HG-epitope, and can be rapidly purified using a Ni2+ column.