COLOR SELECTION WITH A HYGROMYCIN-RESISTANCE-BASED ESCHERICHIA COLI-MYCOBACTERIAL SHUTTLE VECTOR

Authors
HOWARD NS GOMEZ JE KO C BISHAI WR
Citation
Ns. Howard et al., COLOR SELECTION WITH A HYGROMYCIN-RESISTANCE-BASED ESCHERICHIA COLI-MYCOBACTERIAL SHUTTLE VECTOR, Gene, 166(1), 1995, pp. 181-182
Citations number
8
Categorie Soggetti
Genetics & Heredity
Journal title
Gene → ACNP
ISSN journal
03781119
Volume
166
Issue
1
Year of publication
1995
Pages
181 - 182
Database
ISI
SICI code
0378-1119(1995)166:1<181:CSWAHE>2.0.ZU;2-N
Abstract
Hygromycin-resistance (Hy(R))-based Escherichia coli-mycobacterial shu ttle plasmids have high efficiencies of transformation and a broad myc obacterial host range. We have introduced a lacZ alpha (encoding the a lpha-polypeptide fragment of beta-galactosidase (beta Gal))-multiple c loning site cassette into a Hy(R)-based shuttle vector to generate a p lasmid with nine unique cloning sites and the added feature of beta Ga l color selection in appropriate E. coli host strains.