F. Fava et al., AEROBIC DEGRADATION AND DECHLORINATION OF 2-CHLOROPHENOL, 3-CHLOROPHENOL AND 4-CHLOROPHENOL BY A PSEUDOMONAS-PICKETTII STRAIN, Letters in applied microbiology, 21(5), 1995, pp. 307-312
A Gram-negative aerobic bacterium capable of using 2-chlorophenol (2-C
P), 3-chlorophenol (3-CP) and 4-chlorophenol (4-CP) as sole carbon sou
rces was isolated and characterized. The bacterium, designated LD1, wa
s identified to be a Pseudomonas pickettii strain. LD1 was able to tot
ally degrade and dechlorinate 2-CP (initial concentration: 1.51 mmol l
(-1)), 3-CP (initial concentration: 0.57 mmol l(-1)) and 4-CP (initial
concentration: 0.75 mmol l(-1)) within 30, 30 and 40 h of incubation,
respectively, under growing-cell batch conditions. LD1 was also found
to be able to metabolize chlorocatechols in growing- and resting-cell
conditions. This suggests that the bacterium degrades monochloropheno
ls through a chlorocatechol pathway. In addition, LD1 was found to be
capable of readily metabolizing other organic compounds such as phenol
, benzoic acid, hydroxybenzoic acids and hydroquinone. Because of the
broad spectrum of monochlorophenols and organic compounds that LD1 can
degrade, this bacterium appears to have the potential for being succe
ssfully used in the biotreatment of wastewaters and in soil decontamin
ation.