DEVELOPMENT OF FLUORESCENCE-EMITTING ANTIBODY LABELING SUBSTANCE BY NEAR-INFRARED RAY EXCITATION

Indocyanine green succinimidyl esters, near-infrared labeling dyes, we re synthesized. These reagents were indocyanine green derivatives poss essing N-hydroxysuccinimidyl groups capable of reacting with proteins. The absorption maximum of indocyanine green-labeled human IgG was 785 nm, and its fluorescent excitation and emission maxima were 768 nm an d 807 nm, respectively.