CIRCULATING ANTIGENS IN SCHISTOSOMIASIS - DETECTION OF 31 32-KDA PROTEINS IN SERA FROM PATIENTS INFECTED WITH SCHISTOSOMA-JAPONICUM, SCHISTOSOMA-MANSONI, SCHISTOSOMA-HAEMATOBIUM, OR SCHISTOSOMA-INTERCALATUM/

Authors
LI YL IDRIS MA CORACHAN M HAN JJ KIRSCHFINK M RUPPEL A
Citation
Yl. Li et al., CIRCULATING ANTIGENS IN SCHISTOSOMIASIS - DETECTION OF 31 32-KDA PROTEINS IN SERA FROM PATIENTS INFECTED WITH SCHISTOSOMA-JAPONICUM, SCHISTOSOMA-MANSONI, SCHISTOSOMA-HAEMATOBIUM, OR SCHISTOSOMA-INTERCALATUM/, Parasitology research, 82(1), 1996, pp. 14-18
Citations number
20
Categorie Soggetti
Parasitiology
Journal title
Parasitology research → ACNP
ISSN journal
09320113
Volume
82
Issue
1
Year of publication
1996
Pages
14 - 18
Database
ISI
SICI code
0932-0113(1996)82:1<14:CAIS-D>2.0.ZU;2-Q
Abstract
A sandwich enzyme-linked immunosorbent assay (ELISA) was developed to detect 31/32-kDa schistosome proteins as circulating antigens in sera from schistosomiasis patients. A monoclonal antibody was used as a cap ture antibody and rabbit antiserum raised against purified 31/32-kDa p roteins was the detecting antibody. Positive results were obtained wit h patients infected with Schistosoma japonicum (88%; n=69), S. mansoni (80%; n=56), S. haematobium (100%; n=40), or S. intercalatum (94%; n= 65). Sera from uninfected Chinese and African individuals and from Chi nese patients with trichinosis, cysticercosis, or paragonimiasis did n ot react in the assay. This ELISA appears to be valuable in diagnosing infections by all major human schistosome species.