M. Suhr et al., DIFFERENTIATING STUDIES ON BETA-GALACTOSIDASE ACTIVITY OF HOST AND MICROFLORA AFTER YOGURT INTAKE, Milchwissenschaft, 50(11), 1995, pp. 629-633
Former investigations proved that intestinal hydrolysis of lactose cou
ld be enhanced by ingestion of fermented dairy products with native mi
crobial beta-galactosidases. In order to measure this additional activ
ity directly and to distinguish accurately between host and microbial
beta-galactosidase activity In the rat small intestinal contents an im
munological procedure was developed. Antibodies against the rat small
intestinal beta-galactosidase were bound to CNBr-activated Sepharose.
This gel was than used to separate the rat intestinal beta-galactosida
ses from processed samples of the rat intestinal contents by affinity-
chromatography. The microbial beta-galactosidase activity can be measu
red separately in the effluate. There was no elution of the host enzym
e.