THE COMPLETE NUCLEOTIDE-SEQUENCE OF ODONTOGLOSSUM RINGSPOT VIRUS (CY-1 STRAIN) GENOMIC RNA

The complete nucleotide sequence of the genomic RNA of odontoglossum r ingspot virus Cy-1 strain (ORSV Cy-1) was determined using cloned cDNA . This sequence is 6611 nucleotides long containing four open reading frames, which correspond to 126 K, 183 K, 31 K, and 18 K proteins, Its genomic organization is similar to other tobamoviruses, TMV-V(vulgare ), TMV-H, (tomato strain), tobacco mild green mosaic virus (TMGMV) and cucumber green mottle mosaic virus (CGMMV). The 5' non-coding regions of ORSV Cy-1 is 62 nucleotides. The ORFs encoded a 126 K polypeptide and a 183 K read-through product in which helicase-sequence and polyme rase-sequence motifs are found, The ORFs encoding the 126 K and 183 K proteins have 61% and 63% identities with those of TMV-V. The third OR F encoded a 31 K protein homologous to TMV cell-to-cell movement prote in. It has 63% identities with that of TMV-V. The fourth ORF encoded a n 18 K coat protein. The 5' non-coding region, which extends from base I to 62 has 2 G residues and a ribosome binding site (AUU). The 3' no n-coding region, 414 nucleotides in length, is entirely different from that of other tobamoviruses.