RAPID AND SENSITIVE PCR DETECTION OF VIBRIO-TRACHURI PATHOGENIC TO JAPANESE HORSE MACKEREL (TRACHURUS-JAPONICUS)

A polymerase chain reaction (PCR) method was performed for rapid and s ensitive detection of pathogenic Vibrio trachuri isolated from culture d Japanese horse mackerel, A set of primers was selected from the base sequence of the Pst I fragment of T9210 chromosomal DNA and used for PCR detection of T9210. This PCR specifically amplified the DNAs from V. trachuri T92BO, T9213, and T9216 but not of those other bacterial s trains. PCR using a Pst I-1 primer set made it possible to detect 100 fg of T9210 DNA. The PCR method reported here may be useful for defect ion and identification of V. trachuri pathogenic to Japanese horse mac kerel.