THE ENTRY OF REOVIRUS INTO L-CELLS IS DEPENDENT ON VACUOLAR PROTON-ATPASE ACTIVITY

Inhibitors of vacuolar proton-ATPase activity (5 mu M bafilomycin Al o r 50 nM concanamycin A) prevented infection by reovirus particles but not by infectious subviral particles (ISVPs), Neither compound affecte d virus attachment or internalization. However, both compounds potentl y blocked cleavage of the viral protein mu 1C. Finally, both reovirus particles and ISVPs efficiently translocated the toxin alpha-sarcin to the cytosol during virus entry. Bafilomycin Al blocked translocation of alpha-sarcin by reovirus particles but not by ISVPs.