MOLECULAR-CLONING OF THE HUMAN GENE SUVCC3 ASSOCIATED WITH THE FORMATION OF DNA-PROTEIN CROSS-LINKS FOLLOWING EXPOSURE TO SOLAR UV-RADIATION

DRP 153 cells, which are hypersensitive to solar Wand deficient in the formation of DNA-protein crosslinks (DPC) following irradiation, were transfected with human DNA and a secondary transformant obtained in w hich a normal DPC response and solar UV sensitivity reestablished. DNA from this secondary transformant was used to construct a genomic DNA library from which a recombinant phage was isolated containing the hum an gene capable of restoring a normal DPC response and solar UV sensit ivity to DRP 153. This gene has been designated SUVCC3 to denote solar UV cross-complementing gene number 3.