CHROMOSOMAL LOCALIZATION AND EXPRESSED SEQUENCE TAG GENERATION OF CLONES FROM A NORMALIZED HUMAN ADULT THYMUS CDNA LIBRARY

Expressed sequence tags (ESTs) from 298 clones have been generated fro m a randomly primed, normalized human adult thymus cDNA library. We de scribe the chromosomal localization of 136 of these ESTs by PCR-based mapping to a human monochromosomal somatic cell hybrid panel. Data bas e similarities to known genes are also described. A subset (n = 18) of these randomly primed ESTs extended the sequence of ESTs from other t issues currently in dbEST. OF the nonrepetitive human adult thymus EST s generated in this study, 237 (79.5%) have no similarity to current d ata base entries. This would suggest that our collection contains -100 new coding regions from thymus tissue, a large proportion of which li kely will represent the middle regions of genes. The mapped ESTs shoul d prove useful as new gene-based markers for mapping and candidate gen e hunting, particularly when anchored to a well-developed physical map of the human genome.