A CARBENE-GENERATING BIOTINYLATED LACTOSYLCERAMIDE ANALOG AS NOVEL PHOTOREACTIVE SUBSTRATE FOR GM(3) SYNTHASE

Authors
HATANAKA Y HASHIMOTO M HIDARI KIPJ SANAI Y NAGAI Y KANAOKA Y
Citation
Y. Hatanaka et al., A CARBENE-GENERATING BIOTINYLATED LACTOSYLCERAMIDE ANALOG AS NOVEL PHOTOREACTIVE SUBSTRATE FOR GM(3) SYNTHASE, Bioorganic & medicinal chemistry letters, 5(23), 1995, pp. 2859-2862
Citations number
15
Categorie Soggetti
Chemistry Inorganic & Nuclear","Chemistry Medicinal
Journal title
Bioorganic & medicinal chemistry letters → ACNP
ISSN journal
0960894X
Volume
5
Issue
23
Year of publication
1995
Pages
2859 - 2862
Database
ISI
SICI code
0960-894X(1995)5:23<2859:ACBLAA>2.0.ZU;2-C
Abstract
A new biotinylated lactose derivative bearing a C-14-labeled phenyldia zirine was synthesized. A convenient approach based on avidin-biotin t echnology was successfully applied for GM(3) synthase assay and the Km value of this biotinylated photoprobe was determined as 180 mu M usin g rat liver Golgi as the enzyme source. Further characterization revea led that this reagent could be a useful photoprobe for GM(3) synthase.