T. Matsunaga et al., DIRECT COUNT OF BACTERIA USING FLUORESCENT DYES - APPLICATION TO ASSESSMENT OF ELECTROCHEMICAL DISINFECTION, Analytical chemistry, 67(24), 1995, pp. 4487-4490
A rapid method for direct counting of viable and dead cells using two
fluorescent dyes, propidium iodide and 4',6-diamidino-2-phenylindole,
was developed for estimation of cell viability after electrochemical d
isinfection. Using this double staining method, dead and viable cells
were visualized as red and blue fluorescent cells, respectively, under
UV excitation employing epifluorescence microscopy. A good correlatio
n between the red cell ratio and the ratio of dead cells determined by
the plate count method was obtained for Vibrio alginolyticus cells ex
posed to heat (60 degrees C), 10 ppm of chlorine, 3.7% of formaldehyde
, and electrochemical disinfection treatment.