MICROSCALE FLOW-INJECTION AND MICROBORE HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY COUPLED WITH INDUCTIVELY-COUPLED PLASMA-MASS SPECTROMETRY VIA A HIGH-EFFICIENCY NEBULIZER
Sa. Pergantis et al., MICROSCALE FLOW-INJECTION AND MICROBORE HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY COUPLED WITH INDUCTIVELY-COUPLED PLASMA-MASS SPECTROMETRY VIA A HIGH-EFFICIENCY NEBULIZER, Analytical chemistry, 67(24), 1995, pp. 4530-4535
A high-efficiency nebulizer has been used for coupling microscale now
injection and microbore high-performance liquid chromatography with in
ductively coupled plasma mass spectrometry (ICPMS). The microscale now
injection system was configured to minimize band broadening between t
he injection valve and the nebulizer, and it was evaluated for now rat
es between 20 and 120 mu L/min. Various materials, including certified
reference materials, were analyzed for their arsenic and lead content
s, Separation of arsenic species was carried out on microbore liquid c
hromatography columns. The absolute detection limits (femtogram range)
for various arsenic compounds were excellent compared with those obta
ined previously by using conventional column (1 mL/min eluent now) hig
h-performance liquid chromatography/inductively coupled plasma mass sp
ectrometry, Other advantages of this system include use of small sampl
e volumes (less than or equal to 1 mu L), the resulting minimization o
f sample matrix introduction into the plasma, and reduced waste genera
tion compared with conventional ICPMS sampling systems.