STRUCTURE OF AN ACIDIC POLYSACCHARIDE FROM THE LYSOAMIDASE BACTERIOLYTIC COMPLEX

The structure of an acidic polysaccharide from the lysoamidase bacteri olytic complex was studied. On the basis of sugar analysis of the init ial and carboxyl-reduced polysaccharide and H-1- and C-13-NMR spectros copy of the initial and O-deacetylated polysaccharide, including two-d imensional NMR spectroscopy, the following structure of the trisacchar ide repeating unit of the polysaccharide was established: [GRAPHICS] w here ManNAcA and GalNAcA are 2-acetamido-2-deoxymannuronic acid and 2- acetamido-2-deoxygalacturonic acid, respectively. The lysoamidase comp lex contains also a small amount of a neutral polysaccharide consistin g of rhamnose.