A SCHEME FOR PURIFICATION OF ENZYMES OF THE CELLULASE COMPLEX OF PENICILLIUM-VERRUCULOSUM AND INVESTIGATION OF THEIR BIOCHEMICAL-PROPERTIESAND SPECIFICITIES

A scheme for isolation and purification of cellulolytic enzymes from P enicillium verruculosum is suggested which includes purification of th e proteins as a group by ammonium sulfate precipitation, ion-exchange column chromatography on DEAE-Spheron, and chromatofocusing in a pH gr adient followed by fine enzyme separation by high performance ion-exch ange column chromatography on Macro prep Q. Five endoglucanases and tw o cellobiohydrolases which are homogeneous by analytical isoelectric f ocusing and gel chromatography were isolated. The molecular weights an d isoelectric points of these enzymes varied from 19,100-114,800 dalto ns and 3.70-4.38, respectively. The carbohydrate contents are 8.1-48.3 %. The activities of the isolated cellulase complex enzymes toward var ious cellulose species and substrates with different glycoside bond ty pes were determined.