J. Craigo et al., UTERINE CERVIX ADENOCARCINOMA WITH BOTH HUMAN PAPILLOMAVIRUS TYPE-18 AND TUMOR-SUPPRESSOR GENE P53 MUTATION FROM A WOMAN HAVING AN INTACT HYMEN, Gynecologic oncology, 59(3), 1995, pp. 423-426
Human papillomavirus (HPV) type 18 DNA was found in an aggressively in
vasive adenocarcinoma tumor from a woman who had an intact hymen and d
enied any prior sexual intercourse, The viral DNA was detected within
the tumor tissue by polymerase chain reaction (PCR) using consensus pr
imers for the L1 region of oncogenic high-risk genital HPVs. In order
to determine mutations within the tumor suppressor gene p53, the gene
exons were amplified by PCR followed by single-stranded conformation p
olymorphism (SSCP) analysis, The appearance of a mutation in exon 8 of
the p53 gene suggested by SSCP was directly confirmed by DNA sequenci
ng of the exon, The sequencing showed a single base deletion that may
truncate the protein by introducing a early stop codon in the messenge
r RNA, This early truncation could be expected to affect the proper ol
igomerization of the p53 protein and hence its DNA-binding activity, T
he results show that genital oncogenic human papillomaviruses may be p
assed by nonsexual routes and suggest that the virus may work in conce
rt with p53 mutations to help the infected tissue progress toward inva
sive cancer. (C) 1995 Academic Press, Inc.