EVALUATION OF A SPATIAL SCANNING LASER FLUOROMETRIC DETECTOR FOR CAPILLARY ELECTROPHORESIS - APPLICATION TO STUDIES OF BAND MIGRATION AND DISPERSION

We report herein the development and evaluation of modified capillary electrophoresis instrumentation with laser fluorometric detection whic h allows for the convenient and precise translation of the detection z one along the length of the separation column. This instrumentation is utilized for fundamental studies of band dispersion, analyte mobility alterations, sample stacking, and the determination of diffusion coef ficients. The feasibility of using this instrumentation to improve the signal/noise ratio and decrease analysis time is also presented. DNA restriction digest separations employing soluble polymer buffer system s with on-column fluorescent labeling was used to illustrate the uniqu e advantages of this experimental design. (C) 1995 John Wiley & Sons, Inc.