In sapwood challenge experiments in Acer rubrum, columns of discoloura
tion initiated by wounding and inoculation with pioneer fungi (Cephalo
sporium sp., Phialophora sp.) were similar in size to untreated wounds
. Inoculation with decay fungi (Pleurotus ostreatus, Trametes versicol
or) produced larger columns of wound-initiated discolouration. The rem
oval of bark around a bore wound caused a significantly larger column
to form compared to the sum of the columns initiated by separate wound
s. Stage-I discoloured wood, nor associated with obviously rotted wood
, had concentrations of mobile cations and soluble phenols similar to
sapwood. Stage-II discoloured wood, spatially associated with rotted w
ood, was frequently bounded by a chemically distinct boundary la;er an
d the discoloured wood contained significantly greater concentrations
of mobile cations and soluble phenols than stage-I discoloured wood.