ANALYSIS OF POLYMERASE CHAIN-REACTION PRODUCT BY CAPILLARY ELECTROPHORESIS AND ITS APPLICATION TO THE DETECTION OF SINGLE-BASE SUBSTITUTIONIN GENES

Capillary gel electrophoresis (CGE) was studied for the direct analysi s of polymerase chain reaction (PCR) amplified samples. A low cross-li nked polyacrylamide gel (3%T, 0.5%C) was used for CGE with treated and untreated silica capillaries. CGE showed high reproducibility and res olution in the separation of DNA fragments (ca. 100-1000 base pairs) p roduced by PCR. The CGE system was applied to the detection of an ampl ification refractory mutation system (ARMS) and PCR-restriction fragme nt length polymorphism (PCR-RFLP), which are detection methods of sing le base substitution in genes using PCR. With the CGE system, full aut omation of PCR product detection is feasible.