FLOW-INJECTION ANALYSIS WITH SIMPLE CHEMILUMINESCENCE DETECTOR FOR HYPOXANTHINE

A low cost chemiluminescence detector with a photodiode for flow injec tion analysis (FIA) of hypoxanthine in fish and meat was developed for quality control in food industry. In this system, hypoxanthine is oxi dized enzymatically to produce hydrogen peroxide during passage throug h the xanthine oxidase immobilized reactor, Then, chemi-luminescence c aused by mixing hydrogen peroxide with chemiluminescence reagent was d etected by the now through photodiode cell that was designed to detect efficiently weak chemiluminescence. This light intensity was proporti onal to hypoxanthine concentration, As a result, a linear working curv e was obtained from 2 x 10(-7) to 2 x 10(-4) mol l(-1) hypoxanthine co ncentration in this FIA system, The present system with the photodiode detector was applied to the determination of hypoxanthine in fish and meat samples. The result:, were compared with those obtained by the c onventional HPLC method, Analytical results for fish samples obtained by the present FIB method were fairly in good agreement with those obt ained by the conventional HPLC method, Correlation factor and regressi on line between both methods were 0.978 and Y = 0.89X + 0.66 respectiv ely, The present FIA system was concluded to be available as a simple, easy-handling and reliable instrument for quality control in food ind ustry.