ELECTROCHEMICAL CHARACTERIZATION OF GALACTOSE-OXIDASE COUPLED WITH FERROCENE DERIVATIVES

DC-cyclic voltammetry was employed for characterization of an active s ite of galactose oxidase(GAO) coupled with some electrode-active media tors and alkaline shift of the pH dependence in electrocatalytic react ion. We discussed on difference of the reactivity of various mediators with native and modified GAG. (Ferrocenylmethyl)trimethylammonium per chlorate gave the highest catalytic reactivity in ferrocene derivative s used as mediators of GAG. These electrocatalytic reactions were esti mated to be related with negative charge near the active site of GAG. Moreover alkaline shift of the electrochemical behavior was caused by a local increase of proton which was produced during oxidation of a su bstrate.