DIRECT MEASUREMENT OF ENANTIOMERIC EXCESS OF SYNTHETIC TERT-LEUCINE WITH A CHIRAL-LIGAND-EXCHANGE CHROMATOGRAPHY

Citation
Sh. Wang et al., DIRECT MEASUREMENT OF ENANTIOMERIC EXCESS OF SYNTHETIC TERT-LEUCINE WITH A CHIRAL-LIGAND-EXCHANGE CHROMATOGRAPHY, Journal of the Chinese Chemical Society, 42(6), 1995, pp. 953-956
Citations number
8
Categorie Soggetti
Chemistry
ISSN journal
00094536
Volume
42
Issue
6
Year of publication
1995
Pages
953 - 956
Database
ISI
SICI code
0009-4536(1995)42:6<953:DMOEEO>2.0.ZU;2-9
Abstract
Racemic tert-leucine can be completely resolved by ligand exchange chr omatography (LEG) using a Chiralpak WH column. The retention time of t he tert-leucine was decreased by increasing concentration of Cu2+, or decreasing pH of the eluent whereas it was unaffected by varying the t emperature of eluent. Optimal conditions for complete separation of th e D,L-tert-leucine were found to be (Cu2+, 5.0 mM, PH 5.5, 25 degrees C). The enantiomeric excess of synthetic tert-leucine, a chiral auxili ary reagent, was determined.