GLUCOSE-TRANSPORT BY THE PHOSPHOENOLPYRUVATE - MANNOSE PHOSPHOTRANSFERASE SYSTEM IN LACTOBACILLUS-CASEI ATCC-393 AND ITS ROLE IN CARBON CATABOLITE REPRESSION

A 2-deoxy-D-glucose-resistant mutant of a pLZ15-cured derivative of La ctobacillus casei ATCC 393 was isolated on agar medium containing 10 m M 2-deoxy-D-glucose and 5 g lactose l(-1). The mutant was impaired in the main glucose transport mechanism, a PTSman-type system. Additional ly a proton-motive-force-dependent glucose permease was detected. The growth response and the sugar consumption rates of the wild-type and t he PTSman-deficient mutant suggested that the mutated element of the c omplex IIABC(man) was, in the wild-type, responsible for a strong repr ession by glucose and mannose of the lactose and ribose assimilation g enes, while assimilation of galactose was only weakly repressed. It is postulated that they are regulated by a different mechanism of catabo lite repression.