RECA MUTATIONS REDUCE ADHERENCE AND COLONIZATION BY CLASSICAL AND EL-TOR STRAINS OF VIBRIO-CHOLERAE

Authors
KUMAR KK SRIVASTAVA R SINHA VB MICHALSKI J KAPER JB SRIVASTAVA BS
Citation
Kk. Kumar et al., RECA MUTATIONS REDUCE ADHERENCE AND COLONIZATION BY CLASSICAL AND EL-TOR STRAINS OF VIBRIO-CHOLERAE, Microbiology, 140, 1994, pp. 1217-1222
Citations number
25
Categorie Soggetti
Microbiology
Journal title
Microbiology → ACNP
ISSN journal
13500872
Volume
140
Year of publication
1994
Part
5
Pages
1217 - 1222
Database
ISI
SICI code
1350-0872(1994)140:<1217:RMRAAC>2.0.ZU;2-#
Abstract
Two recA mutants of Vibrio cholerae (classical and El Tor biotypes) we re constructed by disruption of the wild-type recA gene with mutated r ecA sequences of V. cholerae cloned in the suicide vector pGP704. Muta nts defective in the recA gene were compared with their respective Rec A(+) parent strains with regard to their adherence to isolated rabbit intestine and Maryland, School of colonization of intestine of infant mice. The recA mutation in V. cholerae was Medicine, Baltimore, found to diminish adherence and markedly affected colonization.