NUCLEOTIDE-SEQUENCE OF THE GDS1 GENE OF SACCHAROMYCES-CEREVISIAE

We have cloned and sequenced the GDS1 gene located on the right arm of chromosome XV of Saccharomyces cerevisiae. The gene codes for a 522 a mino acid serine-rich protein with no obvious homology to proteins in the database. GDS1 gene was isolated as the multicopy suppressor of th e glycerol-deficient phenotype caused by the nam9-1 mutation in the ye ast nuclear gene encoding the mitochondrial ribosomal protein homologo us to S4 proteins from various organisms. Disruption-deletion of the G DS1 open reading frame leads to a partial impairment of growth on medi um containing glycerol as the carbon source, indicating mitochondrial function of the gene product. The sequence has been deposited in the G enBank data library under Accession Number U18262.