CHARACTERIZATION OF A SPECIFIC MONOCLONAL-ANTIBODY 9F5-3A AND THE DEVELOPMENT OF ASSAY SYSTEM FOR OXIDIZED HDL

We obtained a monoclonal antibody 9F5-3a against oxidative low-density lipoprotein (LDL) modified with CuSO4 and established a sandwich ELIS A for detection of oxidized high-density lipoprotein (oxHDL). The 9F5- 3a was reacted strongly with oxHDL and to a lesser degree with oxLDL a nd LDL. In contrast, little or no reactivity was found with HDL. When the generation of oxHDL was limited by the addition of alpha-tocophero l and catalase, reactivity to 9F5-3a was reduced. Incubation of oxHDL with excess lyso-phosphatidylcholine (lyse-PC) also reduced immunoreac tivity, but not by only lyse-PC. These results suggested that the epit ope is possibly associated with oxHDL-linked lyse-PC induced mainly by the hydroxyl radical. (C) 1995 Academic Press, Inc.