MOLECULAR-CLONING OF A NOVEL MACROPHAGE MATURATION-ASSOCIATED TRANSCRIPT ENCODING A PROTEIN WITH SEVERAL POTENTIAL TRANSMEMBRANE DOMAINS

Citation
M. Rehli et al., MOLECULAR-CLONING OF A NOVEL MACROPHAGE MATURATION-ASSOCIATED TRANSCRIPT ENCODING A PROTEIN WITH SEVERAL POTENTIAL TRANSMEMBRANE DOMAINS, Biochemical and biophysical research communications, 217(2), 1995, pp. 661-667
Citations number
24
Categorie Soggetti
Biology,Biophysics
ISSN journal
0006291X
Volume
217
Issue
2
Year of publication
1995
Pages
661 - 667
Database
ISI
SICI code
0006-291X(1995)217:2<661:MOANMM>2.0.ZU;2-W
Abstract
Differentiation of tissue macrophages from bone marrow progenitor cell s via circulating blood monocytes is a complex and multistep process. In order to analyze macrophage maturation on the molecular level we us ed the method of mRNA differential display to identify novel genes tha t are preferentially expressed in mature, in vitro differentiated macr ophages. A PCR-fragment could be isolated that was amplified from macr ophages, but not from freshly isolated monocytes, and macrophage-speci fic expression was confirmed by Northern blot analysis. Several corres ponding cDNA clones could be isolated from a macrophage cDNA library, covering a nucleotide sequence of 2.5 kb. Nucleotide sequence of this cDNA showed no homology to known genes. The sequence reveals an open r eading frame that codes for a 238 amino acid hydrophobic protein with seven potential transmembrane domains, suggesting a possible role as a receptor or an ion channel. (C) 1995 Academic Press, Inc.