CYTOCIDAL INFECTION OF HOG-CHOLERA VIRUS IN PORCINE BONE-MARROW STROMA CELL-CULTURES

Porcine bone marrow stroma cell (BMSC) cultures producing cells of gra nulocyte-lineage were established. Hog cholera (HC) virus ALD and Alfo rt strains replicated in the porcine BMSC cultures showing distinct cy topathic effect (CPE). The differentiation of granulocyte-lineage cell s in the cultures ceased after infection with HC virus. Polyclonal ant ibody against the ALD strain inhibited completely the development of C PE of the both ALD and Alfort strains. Monoclonal antibodies (mAbs) sp ecific to the ALD strain inhibited CPE of the ALD strain, while CPE of the Alfort strain was not affected by those mAbs, suggesting that CPE induced in the BMSC cultures is due to HC virus.