Fresh human gastrointestinal cancer cells are more resistant to antica
ncer drugs compared to other cancer cells, and the selection of antica
ncer drugs for cancer chemotherapy is important. In the present study,
it is demonstrated that MTX enhanced the chemosensitivity of 5-FU, es
pecially, in the tumor cells with less than 70% inhibition ratio by th
e MTT assay. It has been reported that MTX/5-FU sequential chemotherap
y was one of the effective chemotherapies against gastric cancer and c
olon cancer, and it is possible to anticipate the efficacy of MTX/5-FU
sequential chemotherapy by the MTT assays.