DETERMINATION OF FENOVERINE IN TISSUE SAMPLES BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY

Fenoverine is a spasmolytic, non-anticholinergic phenothiazine derivat ive that inhibits calcium channel currents. We describe a high-perform ance liquid chromatographic method for the determination of fenoverine in striated muscle, smooth, muscle, myocardium, and liver tissue. Rev ersed-phase liquid chromatography was performed on a 5 mum Nucleosil C 18 column with acetonitrile-0.015 M phosphate buffer (28:72, v/v) as a mobile phase and detection with ultraviolet at 214 nm. The limit of q uantitation of fenoverine in tissue samples was 25 ng injected. This m ethod is well suited for the determination of fenoverine in various or gans in animal experiments.