Jc. Jacquier et Pl. Desbene, DETERMINATION OF CRITICAL MICELLE CONCENTRATION BY CAPILLARY ELECTROPHORESIS - THEORETICAL APPROACH AND VALIDATION, Journal of chromatography, 718(1), 1995, pp. 167-175
Citations number
11
Categorie Soggetti
Chemistry Analytical","Biochemical Research Methods
The aim of this study was the determination of the critical micelle co
ncentration (CMC) of the most commonly employed anionic surfactant in
micellar electrokinetic chromatography, sodium dodecyl sulphate (SDS),
using capillary electrophoresis, i.e., under the operating conditions
of the electrophoretic separation (pH, ionic strength, temperature, e
tc.). The effective electrophoretic mobility of a neutral compound, re
sulting from the solvophobic and micellar contributions, appeared to b
e a well adapted parameter for the study of the micellization process
of anionic surfactants. In fact, the theoretical treatment of the evol
ution of this parameter as a function of the total surfactant concentr
ation allowed the identification of a sharp change in slope at the cmc
, and therefore to establish capillary electrophoresis as a new analyt
ical tool for the determination of critical micelle concentrations. In
the experimental case of an electrolytic solution consisting of 5 mmo
l l(-1) borax, the observed value of the cmc of SDS (5.29 mmol l(-1))
was in total agreement with the literature data when the sodium concen
tration in the solution was the same.